pMXs_FLAG-MCART1
(Plasmid
#133247)
-
PurposeExpress FLAG-tagged MCART1 in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 133247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMXs
-
Backbone manufacturerCell Biolabs, Inc.
- Backbone size w/o insert (bp) 5600
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMCART1
-
Alt nameSLC25A51
-
SpeciesH. sapiens (human)
-
Mutationcodon-optimized for expression in human cells and PAM sequence mutated
-
Entrez GeneSLC25A51 (a.k.a. CG7943, MCART1)
-
Tag
/ Fusion Protein
- 3xFLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer GTGGACCATCCTCTAG
- 3′ sequencing primer GACGGCAATATGGTG (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMXs_FLAG-MCART1 was a gift from David Sabatini (Addgene plasmid # 133247 ; http://n2t.net/addgene:133247 ; RRID:Addgene_133247) -
For your References section:
MCART1/SLC25A51 is required for mitochondrial NAD transport. Kory N, Uit de Bos J, van der Rijt S, Jankovic N, Gura M, Arp N, Pena IA, Prakash G, Chan SH, Kunchok T, Lewis CA, Sabatini DM. Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. Print 2020 Oct. 10.1126/sciadv.abe5310 PubMed 33087354