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Addgene

MOM603
(Plasmid #133242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcebac1
  • Total vector size (bp) 9087
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kif1a
  • Alt name
    kinesin superfamily protein 1A
  • Alt name
    SPG30
  • Alt name
    ATSV
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5323
  • GenBank ID
    AB210029
  • Entrez Gene
    KIF1A (a.k.a. ATSV, C2orf20, HSN2C, MRD9, NESCAVS, SPG30, UNC104)
  • Promoter polyhedrin promoter
  • Tags / Fusion Proteins
    • mScarlet (C terminal on insert)
    • StrepII tag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAATGATAACCATCTCGC
  • 3′ sequencing primer ATTTTATGTTTCAGGTTCAGGGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MOM603 was a gift from Shinsuke Niwa (Addgene plasmid # 133242 ; http://n2t.net/addgene:133242 ; RRID:Addgene_133242)

  • For your References section:

    Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors. Chiba K, Takahashi H, Chen M, Obinata H, Arai S, Hashimoto K, Oda T, McKenney RJ, Niwa S. Proc Natl Acad Sci U S A. 2019 Aug 27. pii: 1905690116. doi: 10.1073/pnas.1905690116. 10.1073/pnas.1905690116 PubMed 31455732
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