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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13324 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKC16
- Backbone size w/o insert (bp) 15500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegyrB
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Alt namegyrase B
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SpeciesEscherichia coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A fragment containing gyrB, recF, dnaN, dnaA, and lambda N genes was trimmed with nuclease Bal31 and then cut with BamHI and inserted into the BamHI site of pKC16. See article for cloning details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK47 was a gift from Martin Gellert (Addgene plasmid # 13324 ; http://n2t.net/addgene:13324 ; RRID:Addgene_13324) -
For your References section:
Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins. Mizuuchi K, Mizuuchi M, O'Dea MH, Gellert M. J Biol Chem. 1984 Jul 25. 259(14):9199-201. PubMed 6086626