pOPS0386
(Plasmid
#133236)
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PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOGG026 (Addgene ID 113992)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameconstructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
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SpeciesSynthetic
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MutationDomesticated for Golden Gate cloning
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NoP-celB-pOGG026.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPS0386 was a gift from Philip Poole (Addgene plasmid # 133236 ; http://n2t.net/addgene:133236 ; RRID:Addgene_133236) -
For your References section:
Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules. Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. 10.1073/pnas.1921225117 PubMed 32317381