pGEX5X.1 PINK1 WT
(Plasmid #13321)

• Depositing Lab: Mark Cookson
• Publication: Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8.

Backbone

• Vector backbone: pGEX5X.1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PINK1 kinase domain
• Species: H. sapiens (human)
• Insert Size (bp): 1152
• Mutation: Amino acids 112-496
• Entrez Gene: PINK1 (a.k.a. BRPK, PARK6)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry