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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti6/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 8688
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePINK1
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SpeciesH. sapiens (human)
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Insert Size (bp)1743
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MutationG309D
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Entrez GenePINK1 (a.k.a. BRPK, PARK6)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6-DEST PINK1-V5 G309D was a gift from Mark Cookson (Addgene plasmid # 13318 ; http://n2t.net/addgene:13318 ; RRID:Addgene_13318) -
For your References section:
Mutations in PTEN-induced putative kinase 1 associated with recessive parkinsonism have differential effects on protein stability. Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller DW, Petsko GA, Cookson MR. Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8. 10.1073/pnas.0500617102 PubMed 15824318