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Addgene

pOPS0520
(Plasmid #133161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133161 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pOGG026 (Addgene ID 113992)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ST18 (S17 delta pir delta hemA)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PU module (PsNifH from pOGG043), SC module (sfGFP from pOGG037), and T module (CAAATTCGGGAT and universal primer binding site)
  • Species
    Synthetic
  • Mutation
    Domesticated for Golden Gate cloning

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The only difference is the sequence: CAAATTCGGGAT .

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPS0520 was a gift from Philip Poole (Addgene plasmid # 133161)

  • For your References section:

    Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules. Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. 10.1073/pnas.1921225117 PubMed 32317381
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