pOPS0514
(Plasmid #133155)

• Purpose: Reporter plasmid with a 12-nucleotide specific "barcode" sequence. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).
• Depositing Lab: Philip Poole
• Publication: Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21.

Backbone

• Vector backbone: pOGG026 (Addgene ID 113992)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ST18 (S17 delta pir delta hemA)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PU module (PsNifH from pOGG043), SC module (sfGFP from pOGG037), and T module (GCAACACCATCC and universal primer binding site)
• Species: Synthetic
• Mutation: Domesticated for Golden Gate cloning

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The only difference is the sequence: GCAACACCATCC . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).