pOPS0501
(Plasmid
#133142)
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PurposeReporter plasmid with a 12-nucleotide specific "barcode" sequence. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOGG026 (Addgene ID 113992)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ST18 (S17 delta pir delta hemA)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePU module (PsNifH from pOGG043), SC module (sfGFP from pOGG037), and T module (ACCGGTATGTAC and universal primer binding site)
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SpeciesSynthetic
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MutationDomesticated for Golden Gate cloning
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The only difference is the sequence: ACCGGTATGTAC . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPS0501 was a gift from Philip Poole (Addgene plasmid # 133142) -
For your References section:
Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules. Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. 10.1073/pnas.1921225117 PubMed 32317381