pCMVTNT PINK1 C-myc
(Plasmid #13314)

• Depositing Lab: Mark Cookson
• Publication: Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8.

Backbone

• Vector backbone: pCMVTNT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4050
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PINK1
• Species: H. sapiens (human)
• Insert Size (bp): 1743
• Entrez Gene: PINK1 (a.k.a. BRPK, PARK6)
• Tag / Fusion Protein:
  • myc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Resource Information

• Addgene Notes:
  • Note
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry