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Addgene

pFUM
(Plasmid #133130)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLGZ920
  • Backbone size w/o insert (bp) 5403
  • Total vector size (bp) 7038
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    fumarase gene
  • Alt name
    fum
  • Species
    Actinobacillus succinogenes
  • Insert Size (bp)
    1685
  • Promoter PEP-carboxykinase promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AATTATCAATGAGGTGAAGTATGACATTTCGTATTGAAAAAGACAC
  • 3′ sequencing primer GAATTCGAGCTCGGTACCCGGGGATCCCTGACCGTCTTCGGTGAATACTGATATAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUM was a gift from Gregg Beckham (Addgene plasmid # 133130 ; http://n2t.net/addgene:133130 ; RRID:Addgene_133130)

  • For your References section:

    Metabolic Engineering of Actinobacillus succinogenes Provides Insights into Succinic Acid Biosynthesis. Guarnieri MT, Chou YC, Salvachua D, Mohagheghi A, St John PC, Peterson DJ, Bomble YJ, Beckham GT. Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00996-17. doi: 10.1128/AEM.00996-17. Print 2017 Sep 1. 10.1128/AEM.00996-17 PubMed 28625987
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