pPCK
(Plasmid #133128)

• Purpose: a recombinant plasmid, expressing pckA PEP-carboxykinase) gene
• Depositing Lab: Gregg Beckham
• Publication: Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00996-17. doi: 10.1128/AEM.00996-17. Print 2017 Sep 1.

Backbone

• Vector backbone: pLGZ920
• Backbone manufacturer: Pil Kim
• Backbone size w/o insert (bp): 5403
• Total vector size (bp): 7226
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PEP-carboxykinase gene
• Alt name: pckA
• Species: Actinobacillus succinogenes
• Insert Size (bp): 1617
• Promoter: PEP-carboxykinase promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCAGCAATAGAGGAAACACGGTTTG
• 3′ sequencing primer: GGATTTGGTACCGTGCCGGCGGCCTAATAACCTG

Resource Information

• Supplemental Documents:
  • pPCK.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry