pOGG043
(Plasmid
#133123)
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PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOGG068
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePsNifH Synthetic consensus nifH promoter
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SpeciesSynthetic
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MutationDomesticated for Golden Gate cloning
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pL0M-PU-PsNifH. Backbone from Invitrogen for synthesized parts.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOGG043 was a gift from Philip Poole (Addgene plasmid # 133123 ; http://n2t.net/addgene:133123 ; RRID:Addgene_133123) -
For your References section:
Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules. Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. 10.1073/pnas.1921225117 PubMed 32317381
