pGEX-Nedd4-N / 2524 JM16
(Plasmid #13312)

• Depositing Lab: Allan Weissman
• Publication: Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-92.

Backbone

• Vector backbone: pGEX-KG
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nedd4
• Species: M. musculus (mouse)
• Mutation: aa1-52 and aa422-777 deleted
• GenBank ID: U96635
• Entrez Gene: Nedd4 (a.k.a. E430025J12Rik, EG639396, Gm7265, KIAA0093, Nedd4-1, Nedd4a)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pGEX 5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

C-terminal truncation generated by digestion and re-ligation into vector. Therefore, the protein will contain vector-derived amino acids at the C-terminus.