pCLASP_mScarlet_CLASP_RGS2membrane
(Plasmid
#133086)
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PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK146
- Total vector size (bp) 8898
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemScarlet-CLASP
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SpeciesS. cerevisiae (budding yeast)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLASP_mScarlet_CLASP_RGS2membrane was a gift from Hana El-Samad (Addgene plasmid # 133086 ; http://n2t.net/addgene:133086 ; RRID:Addgene_133086) -
For your References section:
Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Chen SY, Osimiri LC, Chevalier M, Bugaj LJ, Nguyen TH, Greenstein RA, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H. Cell Syst. 2020 Sep 4. pii: S2405-4712(20)30293-3. doi: 10.1016/j.cels.2020.08.009. 10.1016/j.cels.2020.08.009 PubMed 32898473