pIRES:hTRAAK
(Plasmid
#133080)
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PurposeMammalian expression vector. It will generate the human TRAAK channel full length
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133080 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES2 EGFP
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Backbone manufacturerClonetech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePotassium channel subfamily K member 4 (KCNK4, TRAAK)
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SpeciesH. sapiens (human)
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Entrez GeneKCNK4 (a.k.a. FHEIG, K2p4.1, TRAAK, TRAAK1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV F
- 3′ sequencing primer CMV R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIRES:hTRAAK was a gift from Dan Minor (Addgene plasmid # 133080 ; http://n2t.net/addgene:133080 ; RRID:Addgene_133080) -
For your References section:
Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels. Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL Jr. Neuron. 2014 Dec 17;84(6):1198-212. doi: 10.1016/j.neuron.2014.11.017. Epub 2014 Dec 11. 10.1016/j.neuron.2014.11.017 PubMed 25500157
