pGEX4T2 gp78 G2BR mt / JM26
(Plasmid #13308)

• Depositing Lab: Allan Weissman
• Publication: Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 103(2):341-6.

Backbone

• Vector backbone: pGEX4T2
• Backbone manufacturer: Amersham Biosciences
• Backbone size w/o insert (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gp78
• Alt name: AMFR
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: cDNA corresponds to aa309-643 derived from human peripheral blood leukocytes. Q579A ; R580A ; M581A ; L582G ; V583G ; Q584G
• GenBank ID: NM_001144
• Entrez Gene: AMFR (a.k.a. GP78, RNF45, SPG89)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry