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Addgene

pIRES:hTRAAK(W262S)
(Plasmid #133078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2 EGFP
  • Backbone manufacturer
    clonetech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S
  • Species
    H. sapiens (human)
  • Entrez Gene
    KCNK4 (a.k.a. FHEIG, K2p4.1, TRAAK, TRAAK1)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES:hTRAAK(W262S) was a gift from Dan Minor (Addgene plasmid # 133078 ; http://n2t.net/addgene:133078 ; RRID:Addgene_133078)

  • For your References section:

    Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels. Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL Jr. Neuron. 2014 Dec 17;84(6):1198-212. doi: 10.1016/j.neuron.2014.11.017. Epub 2014 Dec 11. 10.1016/j.neuron.2014.11.017 PubMed 25500157
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