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Addgene

pGEMHE:hTRAAK(Q258K)
(Plasmid #133077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEMHE

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K
  • Species
    M. musculus (mouse)
  • Mutation
    Q258K
  • Entrez Gene
    Kcnk2 (a.k.a. A430027H14Rik, TREK-1)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEMHE:hTRAAK(Q258K) was a gift from Dan Minor (Addgene plasmid # 133077 ; http://n2t.net/addgene:133077 ; RRID:Addgene_133077)

  • For your References section:

    Transmembrane helix straightening and buckling underlies activation of mechanosensitive and thermosensitive K(2P) channels. Lolicato M, Riegelhaupt PM, Arrigoni C, Clark KA, Minor DL Jr. Neuron. 2014 Dec 17;84(6):1198-212. doi: 10.1016/j.neuron.2014.11.017. Epub 2014 Dec 11. 10.1016/j.neuron.2014.11.017 PubMed 25500157
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