pFastBac_His-ZZ-TEV-Lis1-SNAPf
(Plasmid #133073)

• Purpose: Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag
• Depositing Labs: Simon Bullock, Andrew Carter
• Publication: Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi: 10.7554/eLife.21768.

Backbone

• Vector backbone: pFastBac
• Backbone manufacturer: ThermoFisher Scientific
• Backbone size w/o insert (bp): 5261
• Total vector size (bp): 6986
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Lis1
• Alt name: Lissencephaly-1
• Alt name: Platelet-activating factor acetylhydrolase IB subunit alpha
• Alt name: PAF acetylhydrolase 45 kDa subunit
• Species: H. sapiens (human)
• Insert Size (bp): 1725
• GenBank ID: AH006662
• Entrez Gene: PAFAH1B1 (a.k.a. LIS1, LIS2, MDCR, MDS, NudF, PAFAH)
• Tags / Fusion Proteins:
  • 8x His (N terminal on backbone)
  • ZZ (N terminal on backbone)
  • Linker-TEV-linker_TEV (N terminal on insert)
  • SNAPf (C terminal on insert)

Resource Information

• Supplemental Documents:
  • p2340_His-ZZ-TEV-Lis1-SNAPf.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the LIS1::SNAPf expression construct from Baumbach et al. 2017. (eLife 2017;6:e21768 doi: 10.7554/eLife.21768). Simon Bullock carried out the cloning to introduce the SNAPf tag into the original Lis1 plasmid.