pDarmo.CMVT_v1
(Plasmid
#133072)
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Purpose(Empty Backbone) Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133072 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDarmo_v1
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Backbone manufacturerKacper Rogala
- Backbone size (bp) 6152
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Vector typeMammalian Expression ; Entry vector
- Promoter CMVT (Enhanced CMV)
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Selectable markersGentamicin
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Tags
/ Fusion Proteins
- TwinStrep-Flag-3C (N terminal on insert)
- 3C-Flag-TwinStrep (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CGATCTGGCCATACACTT
- 3′ sequencing primer CAGCGTATCCACATAGCGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDarmo.CMVT_v1 was a gift from Kacper Rogala & David Sabatini (Addgene plasmid # 133072 ; http://n2t.net/addgene:133072 ; RRID:Addgene_133072) -
For your References section:
Structural basis for the docking of mTORC1 on the lysosomal surface. Rogala KB, Gu X, Kedir JF, Abu-Remaileh M, Bianchi LF, Bottino AMS, Dueholm R, Niehaus A, Overwijn D, Fils AP, Zhou SX, Leary D, Laqtom NN, Brignole EJ, Sabatini DM. Science. 2019 Oct 25;366(6464):468-475. doi: 10.1126/science.aay0166. Epub 2019 Oct 10. 10.1126/science.aay0166 PubMed 31601708