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pGEX4T2 gp78 CD-m1,2 / JM25
(Plasmid #13307)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13307 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T2
  • Backbone manufacturer
    Amersham Biosciences
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gp78
  • Alt name
    AMFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    cDNA corresponds to aa309-643 derived from human peripheral blood leukocytes. A342V; M467G ; F468G ; P469R ; V476R ; D479V ; L480D
  • GenBank ID
    NM_001144
  • Entrez Gene
    AMFR (a.k.a. GP78, RNF45, SPG89)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T2 gp78 CD-m1,2 / JM25 was a gift from Allan Weissman (Addgene plasmid # 13307 ; http://n2t.net/addgene:13307 ; RRID:Addgene_13307)

  • For your References section:

    The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B, Mariano J, Tsai YC, Chan AH, Cohen M, Weissman AM. Proc Natl Acad Sci U S A. 2006 Jan 10. 103(2):341-6. 10.1073/pnas.0506618103 PubMed 16407162
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