pMSCV-IRES-GFP-CD4-TLR4 cyto
(Plasmid
#133064)
-
PurposeRetroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 133064 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMSCV-IRES-GFP
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCD4-TLR4cyto
-
SpeciesM. musculus (mouse)
-
Entrez GeneTlr4 (a.k.a. Lps, Ly87, Ran/M1, Rasl2-8)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMSCV-IRES-GFP-CD4-TLR4 cyto was a gift from Jonathan Kagan (Addgene plasmid # 133064 ; http://n2t.net/addgene:133064 ; RRID:Addgene_133064) -
For your References section:
Mechanisms of Toll-like Receptor 4 Endocytosis Reveal a Common Immune-Evasion Strategy Used by Pathogenic and Commensal Bacteria. Tan Y, Zanoni I, Cullen TW, Goodman AL, Kagan JC. Immunity. 2015 Nov 17;43(5):909-22. doi: 10.1016/j.immuni.2015.10.008. Epub 2015 Nov 3. 10.1016/j.immuni.2015.10.008 PubMed 26546281