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Addgene

pMSCV-IRES-hCD2-CD14 (4R)
(Plasmid #133062)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133062 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-IRES-hCD2
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    hCD2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD14 (4R)
  • Species
    M. musculus (mouse)
  • Mutation
    CD14 allele defective for LPS binding (mutations in 4 regulatory regions of CD14: amino acids 26-29;37-40;50-54;73-78)
  • Entrez Gene
    Cd14

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-hCD2-CD14 (4R) was a gift from Jonathan Kagan (Addgene plasmid # 133062 ; http://n2t.net/addgene:133062 ; RRID:Addgene_133062)

  • For your References section:

    Mechanisms of Toll-like Receptor 4 Endocytosis Reveal a Common Immune-Evasion Strategy Used by Pathogenic and Commensal Bacteria. Tan Y, Zanoni I, Cullen TW, Goodman AL, Kagan JC. Immunity. 2015 Nov 17;43(5):909-22. doi: 10.1016/j.immuni.2015.10.008. Epub 2015 Nov 3. 10.1016/j.immuni.2015.10.008 PubMed 26546281
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