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pCI-Neo-gp78 G2BR mt / JM23
(Plasmid #13306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gp78
  • Alt name
    AMFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    Q579A ; R580A ; M581A ; L582G ; V583G ; Q584G
  • GenBank ID
    NM_001144
  • Entrez Gene
    AMFR (a.k.a. GP78, RNF45, SPG89)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    came from f1 cDNA of human gp78 in pCR2.1 from Jun Yokota
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-Neo-gp78 G2BR mt / JM23 was a gift from Allan Weissman (Addgene plasmid # 13306 ; http://n2t.net/addgene:13306 ; RRID:Addgene_13306)

  • For your References section:

    The activity of a human endoplasmic reticulum-associated degradation E3, gp78, requires its Cue domain, RING finger, and an E2-binding site. Chen B, Mariano J, Tsai YC, Chan AH, Cohen M, Weissman AM. Proc Natl Acad Sci U S A. 2006 Jan 10. 103(2):341-6. 10.1073/pnas.0506618103 PubMed 16407162
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