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PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSicoR (pMK1200)
- Backbone size w/o insert (bp) 7810
- Total vector size (bp) 8212
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Vector typeMammalian Expression, Lentiviral
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Selectable markersmRuby2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTau.K18(LM)
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SpeciesH. sapiens (human)
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Insert Size (bp)402
- Promoter EF1A
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Tag
/ Fusion Protein
- mRuby2
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EF-1a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK1254 was a gift from Martin Kampmann (Addgene plasmid # 133057 ; http://n2t.net/addgene:133057 ; RRID:Addgene_133057) -
For your References section:
Compromised function of the ESCRT pathway promotes endolysosomal escape of tau seeds and propagation of tau aggregation. Chen JJ, Nathaniel DL, Raghavan P, Nelson M, Tian R, Tse E, Hong JY, See SK, Mok SA, Hein MY, Southworth DR, Grinberg LT, Gestwicki JE, Leonetti MD, Kampmann M. J Biol Chem. 2019 Oct 2. pii: RA119.009432. doi: 10.1074/jbc.RA119.009432. 10.1074/jbc.RA119.009432 PubMed 31578281
Map uploaded by the depositor.
