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miR290-SE-RGM-tdTomato targeting vector
(Plasmid #133047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1 Topo vector
  • Vector type
    Mouse Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miR290-SE-5arm-RGM-miR290-SE-3arm
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer caggaaacagctatgac
  • 3′ sequencing primer gtaaaacgacggccag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miR290-SE-RGM-tdTomato targeting vector was a gift from Rudolf Jaenisch (Addgene plasmid # 133047 ; http://n2t.net/addgene:133047 ; RRID:Addgene_133047)

  • For your References section:

    Dynamic Enhancer DNA Methylation as Basis for Transcriptional and Cellular Heterogeneity of ESCs. Song Y, van den Berg PR, Markoulaki S, Soldner F, Dall'Agnese A, Henninger JE, Drotar J, Rosenau N, Cohen MA, Young RA, Semrau S, Stelzer Y, Jaenisch R. Mol Cell. 2019 Aug 15. pii: S1097-2765(19)30502-7. doi: 10.1016/j.molcel.2019.06.045. 10.1016/j.molcel.2019.06.045 PubMed 31422875
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