pCAG-mIRFP670nuc-TEV-Xph20-ETWV
(Plasmid #133044)

• Purpose: Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter
• Depositing Lab: Matthieu Sainlos
• Publication: Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi: 10.1038/s41467-019-12528-4.

Backbone

• Vector backbone: pCAG-miRFP670nuc
• Backbone size w/o insert (bp): 7723
• Total vector size (bp): 8140
• Modifications to backbone: Home-made vector derived from pCAG, which produces in frame a mIRFP670 reporter fused to NLS sequences, the TEV protease and the TEV cleavage site followed by the protein of interest.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Xph20-ETWV
• Species: Synthetic
• Insert Size (bp): 417
• Mutation: S63K
• Promoter: CAG
• Tags / Fusion Proteins:
  • miRFP670 (N terminal on backbone)
  • NLS (N terminal on backbone)
  • TEV protease + TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry