SiC-V2-Cas9G7
(Plasmid
#133043)
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PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneTet-pLKO-puro
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTet repressor
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gRNA/shRNA sequenceCas9G7
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Tag
/ Fusion Protein
- Cerulean
Resource Information
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A portion of this plasmid was derived from a plasmid made byDmitri Wiederschain (Addgene plasmid # 21915) and Sriram Neelamegham (Addgene plasmid # 64847)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SiC-V2-Cas9G7 was a gift from Sriram Neelamegham (Addgene plasmid # 133043 ; http://n2t.net/addgene:133043 ; RRID:Addgene_133043) -
For your References section:
Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski AB, Singhi N, Nemeth M, Neelamegham S. Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006. 10.1016/j.ymthe.2019.09.006 PubMed 31601489
