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Addgene

SiC-V1-Scr
(Plasmid #133042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiCRISPR v2
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9
  • gRNA/shRNA sequence
    Scr
  • Species
    Synthetic
  • Tag / Fusion Protein
    • dTomato

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang (Addgene plasmid # 49535), David Schaffer (Addgene plasmid # 42961), and Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54856)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SiC-V1-Scr was a gift from Sriram Neelamegham (Addgene plasmid # 133042 ; http://n2t.net/addgene:133042 ; RRID:Addgene_133042)

  • For your References section:

    Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski AB, Singhi N, Nemeth M, Neelamegham S. Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006. 10.1016/j.ymthe.2019.09.006 PubMed 31601489
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