SiC-V1
(Plasmid
#133041)
-
PurposeSiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneLentiCRISPR v2
-
Vector typeLentiviral, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSpCas9
-
gRNA/shRNA sequenceEmpty
-
SpeciesSynthetic
-
Tag
/ Fusion Protein
- dTomato
Resource Information
-
A portion of this plasmid was derived from a plasmid made byFeng Zhang (Addgene plasmid # 49535), David Schaffer (Addgene plasmid # 42961), and Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54856)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
SiC-V1 was a gift from Sriram Neelamegham (Addgene plasmid # 133041 ; http://n2t.net/addgene:133041 ; RRID:Addgene_133041) -
For your References section:
Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Kelkar A, Zhu Y, Groth T, Stolfa G, Stablewski AB, Singhi N, Nemeth M, Neelamegham S. Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006. 10.1016/j.ymthe.2019.09.006 PubMed 31601489
