SiC-V1
(Plasmid #133041)

• Purpose: SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.
• Depositing Lab: Sriram Neelamegham
• Publication: Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006.

Backbone

• Vector backbone: LentiCRISPR v2
• Vector type: Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpCas9
• gRNA/shRNA sequence: Empty
• Species: Synthetic
• Tag / Fusion Protein:
  • dTomato

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Feng Zhang (Addgene plasmid # 49535), David Schaffer (Addgene plasmid # 42961), and Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54856)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry