pIG-TAT-Xph20-ETWV
(Plasmid
#133040)
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PurposeExpresses TAT-Xph20-ETWV in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIG-TAT
- Backbone size w/o insert (bp) 5469
- Total vector size (bp) 5763
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Modifications to backboneHomemade vector derived from pET-24-a+, engineered to incorporate immediately after the start codon an AviTag, a decahistidine tag .and a Tobacco Etch Virus cleavage site (-ENLYFQS-) in frame with the protein of interest.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameXph20-ETWV
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SpeciesSynthetic
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Insert Size (bp)294
- Promoter T7
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Tags
/ Fusion Proteins
- His8 (N terminal on backbone)
- TAT (N terminal on backbone)
- TEV protease cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter, Forward primer
- 3′ sequencing primer T7 terminator, reverse primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIG-TAT-Xph20-ETWV was a gift from Matthieu Sainlos (Addgene plasmid # 133040 ; http://n2t.net/addgene:133040 ; RRID:Addgene_133040) -
For your References section:
Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules. Rimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, Puente-Munoz V, Chamma I, Gauthereau I, Antoine S, Thibaut C, Tai FWJ, Dartigues B, Grillo-Bosch D, Claverol S, Poujol C, Choquet D, Mackereth CD, Sainlos M. Nat Commun. 2019 Oct 4;10(1):4521. doi: 10.1038/s41467-019-12528-4. 10.1038/s41467-019-12528-4 PubMed 31586061