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Addgene

pbIG-Xph20-Stg
(Plasmid #133035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pbIG
  • Backbone size w/o insert (bp) 5281
  • Total vector size (bp) 5774
  • Modifications to backbone
    Homemade vector derived from pET-24-a+, engineered to incorporate immediately after the start codon an AviTag, a decahistidine tag .and a Tobacco Etch Virus cleavage site (-ENLYFQS-) in frame with the protein of interest.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Xph20-Stg
  • Species
    Synthetic
  • Insert Size (bp)
    493
  • Mutation
    S63K
  • Promoter T7
  • Tags / Fusion Proteins
    • His10 (N terminal on backbone)
    • AviTag (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BlpI (not destroyed)
  • 5′ sequencing primer T7 promoter, Forward primer
  • 3′ sequencing primer T7 terminator, reverse primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pbIG-Xph20-Stg was a gift from Matthieu Sainlos (Addgene plasmid # 133035 ; http://n2t.net/addgene:133035 ; RRID:Addgene_133035)

  • For your References section:

    Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules. Rimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, Puente-Munoz V, Chamma I, Gauthereau I, Antoine S, Thibaut C, Tai FWJ, Dartigues B, Grillo-Bosch D, Claverol S, Poujol C, Choquet D, Mackereth CD, Sainlos M. Nat Commun. 2019 Oct 4;10(1):4521. doi: 10.1038/s41467-019-12528-4. 10.1038/s41467-019-12528-4 PubMed 31586061
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