Skip to main content
Addgene

pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin
(Plasmid #133028)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLVX-EF1alpha-IRES-Puromycin
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 8825
  • Total vector size (bp) 9747
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Golgin84
  • Alt name
    Golgin subfamily A member 5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    189
  • Mutation
    deleted amino acids 1-673
  • GenBank ID
    9950
  • Entrez Gene
    GOLGA5 (a.k.a. GOLIM5, RFG5, ret-II)
  • Promoter Human elongation factor 1 alpha
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer aag cgc gat cac atg gtc ctg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin was a gift from David Andrews (Addgene plasmid # 133028 ; http://n2t.net/addgene:133028 ; RRID:Addgene_133028)

  • For your References section:

    A reference library for assigning protein subcellular localizations by image-based machine learning. Schormann W, Hariharan S, Andrews DW. J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090. 10.1083/jcb.201904090 PubMed 31968357
Related items:
Commonly requested with: