pGENE:EFF-1::mCherry::V5
(Plasmid #133020)

• Purpose: EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells
• Depositing Lab: Benjamin Podbilewicz
• Publication: Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 10.1016/j.cell.2014.02.020.

Backbone

• Vector backbone: pGENE
• Backbone size w/o insert (bp): 4498
• Total vector size (bp): 7411
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EFF-1::mCherry::V5
• Alt name: pOA81
• Species: C. elegans (nematode)
• Insert Size (bp): 2913
• Tag / Fusion Protein:
  • V5; mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SpeI (destroyed during cloning)
• 5′ sequencing primer: pGENE Fw
• 3′ sequencing primer: pGENE Rv

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mohler WA, et al. Dev Cell 2002

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry