pMH-EF1-GFP
(Plasmid
#133018)
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Purpose(Empty Backbone) (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH-EF1-GFP
- Backbone size (bp) 8000
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Modifications to backboneinsertion of Ampicillin resistance gene
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Vector typeMammalian Expression
- Promoter EF1
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-EF1-GFP was a gift from Michael Huen (Addgene plasmid # 133018 ; http://n2t.net/addgene:133018 ; RRID:Addgene_133018) -
For your References section:
Screen identifies DYRK1B network as mediator of transcription repression on damaged chromatin. Dong C, West KL, Tan XY, Li J, Ishibashi T, Yu CH, Sy SMH, Leung JWC, Huen MSY. Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17019-17030. doi: 10.1073/pnas.2002193117. Epub 2020 Jul 1. 10.1073/pnas.2002193117 PubMed 32611815
