pet 3d double tentacle delete CP
(Plasmid #13301)

• Depositing Lab: John Cooper
• Publication: Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Backbone

• Vector backbone: pET- 3d
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)
• Alt name: actin CAP Z double tentacle delete
• Species: G. gallus (chicken)
• Insert Size (bp): 2000
• Mutation: Capping protein alpha 1 (with 28 amino acids deleted in the C-terminus) and beta 1 (with 34 amino acids deleted in the C-terminus)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: nco 1 for alpha subunit (not destroyed)
• 3′ cloning site: bam H1 (not destroyed)
• 5′ sequencing primer: SEQUENCE
• 3′ sequencing primer: SEQUENCE

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The wt Capping protein plasmid was received from T. Obinata . PCR mutagenesis was done to delete the alpha and beta tentacles. Check Wear et al., 2003. for more information on the primers.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Soeno et al., 1998