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pTone-gata2aECE-nsGFP
(Plasmid #132975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTol1 vector
  • Backbone size w/o insert (bp) 3299
  • Total vector size (bp) 4776
  • Vector type
    Unspecified ; Transposon-mediated

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    nuclear localized sfGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    1068
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    • 6x myc (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer not appreciable
  • 3′ sequencing primer not appreciable
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    339

Cloning Information for Gene/Insert 2

  • Cloning method Gateway Cloning
  • 5′ sequencing primer not appreciable
  • 3′ sequencing primer not appreciable
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTone-gata2aECE-nsGFP was a gift from Nathan Lawson (Addgene plasmid # 132975 ; http://n2t.net/addgene:132975 ; RRID:Addgene_132975)

  • For your References section:

    Valves Are a Conserved Feature of the Zebrafish Lymphatic System. Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND. Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26. 10.1016/j.devcel.2019.08.019 PubMed 31564611
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