pCS-foxc1a-2a-nlstdTomato
(Plasmid
#132970)
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Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132970 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS3MT
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5882
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nametdTomato
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SpeciesDiscosoma
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Insert Size (bp)1812
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Tags
/ Fusion Proteins
- myc (N terminal on insert)
- NLS (N terminal on insert)
Gene/Insert 2
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Gene/Insert namefoxc1a
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1428
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Entrez Genefoxc1a (a.k.a. foxc1.1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS-foxc1a-2a-nlstdTomato was a gift from Nathan Lawson (Addgene plasmid # 132970 ; http://n2t.net/addgene:132970 ; RRID:Addgene_132970) -
For your References section:
Valves Are a Conserved Feature of the Zebrafish Lymphatic System. Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND. Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26. 10.1016/j.devcel.2019.08.019 PubMed 31564611