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Addgene

EGFP-plk1-AKAP
(Plasmid #132967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    polo-like kinase 1-AKAP
  • Species
    H. sapiens (human)
  • Mutation
    C terminus of full-length Plk1 fused to the pericentrin-AKAP450 centrosomal targeting domain of AKAP450
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Entrez Gene
    AKAP9 (a.k.a. AKAP-9, AKAP350, AKAP450, CG-NAP, HYPERION, LQT11, MU-RMS-40.16A, PPP1R45, PRKA9, YOTIAO)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-plk1-AKAP was a gift from Michael Yaffe (Addgene plasmid # 132967 ; http://n2t.net/addgene:132967 ; RRID:Addgene_132967)

  • For your References section:

    Functional dynamics of Polo-like kinase 1 at the centrosome. Kishi K, van Vugt MA, Okamoto K, Hayashi Y, Yaffe MB. Mol Cell Biol. 2009 Jun;29(11):3134-50. doi: 10.1128/MCB.01663-08. Epub 2009 Mar 23. 10.1128/MCB.01663-08 PubMed 19307309
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