plk1 T210D
(Plasmid
#132964)
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PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132964 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plk1 T210D was a gift from Michael Yaffe (Addgene plasmid # 132964 ; http://n2t.net/addgene:132964 ; RRID:Addgene_132964) -
For your References section:
Functional dynamics of Polo-like kinase 1 at the centrosome. Kishi K, van Vugt MA, Okamoto K, Hayashi Y, Yaffe MB. Mol Cell Biol. 2009 Jun;29(11):3134-50. doi: 10.1128/MCB.01663-08. Epub 2009 Mar 23. 10.1128/MCB.01663-08 PubMed 19307309