EFF-1::V5
(Plasmid
#132961)
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PurposeExpresses EFF-1 A full length protein in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGENE B
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Backbone manufacturerINVITROGEN
- Backbone size w/o insert (bp) 4654
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEFF-1::V5
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Alt namepOA34
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SpeciesC. elegans (nematode)
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Insert Size (bp)2203
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GenBank ID174334
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer pGENE Fw
- 3′ sequencing primer pGENE Rv (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMohler WA, et al. Dev Cell 2002
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EFF-1::V5 was a gift from Benjamin Podbilewicz (Addgene plasmid # 132961 ; http://n2t.net/addgene:132961 ; RRID:Addgene_132961) -
For your References section:
Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens. Valansi C, Moi D, Leikina E, Matveev E, Grana M, Chernomordik LV, Romero H, Aguilar PS, Podbilewicz B. J Cell Biol. 2017 Mar 6;216(3):571-581. doi: 10.1083/jcb.201610093. Epub 2017 Jan 30. 10.1083/jcb.201610093 PubMed 28137780