EFF-1::V5
(Plasmid #132961)

• Purpose: Expresses EFF-1 A full length protein in mammalian cells
• Depositing Lab: Benjamin Podbilewicz
• Publication: Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581. doi: 10.1083/jcb.201610093. Epub 2017 Jan 30.

Backbone

• Vector backbone: pGENE B
• Backbone manufacturer: INVITROGEN
• Backbone size w/o insert (bp): 4654
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EFF-1::V5
• Alt name: pOA34
• Species: C. elegans (nematode)
• Insert Size (bp): 2203
• GenBank ID: 174334
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: pGENE Fw
• 3′ sequencing primer: pGENE Rv

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Mohler WA, et al. Dev Cell 2002

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry