pYZ714
(Plasmid #132955)

• Purpose: crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe
• Depositing Lab: Jef Boeke
• Publication: Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 10.1093/nar/gkaa329.

Backbone

• Vector backbone: pombe ars1/ura4
• Backbone size w/o insert (bp): 8273
• Total vector size (bp): 13333
• Vector type: CRISPR, Synthetic Biology
• Selectable markers: Spura4

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: 30°C recommended for this large plasmid. 37°C may also be fine for bacteria growth.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dFnCas12a
• Alt name: dFnCpf1
• gRNA/shRNA sequence: N/A
• Species: H. sapiens (human)
• Promoter: adh1
• Tags / Fusion Proteins:
  • nucleoplasmin NLS (C terminal on insert)
  • SV40 NLS (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CAGTGCCCTACAACAACTAAGAAAATGG
• 3′ sequencing primer: GGGGATCGCAAATTAAAGCCTTCGAG

Resource Information

• Supplemental Documents:
  • pYZ714 fba1.gRNA entry vector with SV40-dFnCas12a-Clr4 in pombe with ura4.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry