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Addgene

pYZ673
(Plasmid #132953)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pombe ars1/ura4
  • Backbone size w/o insert (bp) 7376
  • Total vector size (bp) 12436
  • Vector type
    CRISPR, Synthetic Biology
  • Selectable markers
    Spura4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    30°C recommended for this large plasmid. 37°C may also be fine for bacteria growth.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FnCas12a
  • Alt name
    FnCpf1
  • gRNA/shRNA sequence
    N/A
  • Species
    H. sapiens (human)
  • Promoter adh1

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CAGTGCCCTACAACAACTAAGAAAATGG
  • 3′ sequencing primer GGGGATCGCAAATTAAAGCCTTCGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYZ673 was a gift from Jef Boeke (Addgene plasmid # 132953 ; http://n2t.net/addgene:132953 ; RRID:Addgene_132953)

  • For your References section:

    CRISPR-Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference. Zhao Y, Boeke JD. Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 10.1093/nar/gkaa329. 10.1093/nar/gkaa329 PubMed 32374858
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