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Addgene

pET-S9
(Plasmid #132948)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-Sumo
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Sumo tag is removed
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    30S ribosomal subunit protein S9
  • Species
    E.coli
  • Insert Size (bp)
    426
  • Mutation
    E52C
  • Entrez Gene
    rpsI (a.k.a. b3230, ECK3219)
  • Promoter T7
  • Tag / Fusion Protein
    • N-terminal 6xHis-tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-S9 was a gift from James Williamson (Addgene plasmid # 132948 ; http://n2t.net/addgene:132948 ; RRID:Addgene_132948)

  • For your References section:

    Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding. Duss O, Stepanyuk GA, Puglisi JD, Williamson JR. Cell. 2019 Nov 27;179(6):1357-1369.e16. doi: 10.1016/j.cell.2019.10.035. Epub 2019 Nov 21. 10.1016/j.cell.2019.10.035 PubMed 31761533
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