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pCS2+ gcsf
(Plasmid #132947)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132947 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4094
  • Total vector size (bp) 4832
  • Vector type
    mRNA synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gcsf
  • Alt name
    csf3a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    738
  • Entrez Gene
    csf3a (a.k.a. csf3, gcsf)
  • Promoter SP6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The gcsf clone was cloned into a modified pCS2+ vector (pCS2+ TA; PMID: 24918328), a kind gift of Dr. Robert Bryson-Richardson (Monash University).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ gcsf was a gift from Jason Berman (Addgene plasmid # 132947 ; http://n2t.net/addgene:132947 ; RRID:Addgene_132947)

  • For your References section:

    Stress hematopoiesis induces a proliferative advantage in TET2 deficiency. Rajan V, Collett K, Woodside R, Prykhozhij SV, Moksa M, Carles A, Wong M, Liebman M, Hirst M, Berman JN. Leukemia. 2021 Sep 30. pii: 10.1038/s41375-021-01427-7. doi: 10.1038/s41375-021-01427-7. 10.1038/s41375-021-01427-7 PubMed 34588613
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