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Addgene

ABE7.10-F148A
(Plasmid #132946)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132946 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    X330-U6-Chimeric_BB-CBh-hSpCas9
  • Backbone manufacturer
    Feng Zhang Lab (Addgene#: 42230)
  • Vector type
    Mammalian Expression, CRISPR ; Base editor

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE7.10(F148A)
  • gRNA/shRNA sequence
    Gaacacaaagcatagactgc
  • Species
    Synthetic
  • Insert Size (bp)
    7982
  • Mutation
    ABE7.10(F148A)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ABE7.10-F148A was a gift from Hui Yang (Addgene plasmid # 132946 ; http://n2t.net/addgene:132946 ; RRID:Addgene_132946)

  • For your References section:

    Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Zhou C, Sun Y, Yan R, Liu Y, Zuo E, Gu C, Han L, Wei Y, Hu X, Zeng R, Li Y, Zhou H, Guo F, Yang H. Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10. 10.1038/s41586-019-1314-0 PubMed 31181567
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