pUC57mini ZM249M P90A _env eGFP
(Plasmid
#132939)
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PurposepUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 132939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57mini ZM249M _env eGFP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHIV-1ZM249M provirus (P90A) with env deletion, GFP
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SpeciesHIV
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MutationP90A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SphI (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC57mini ZM249M P90A _env eGFP was a gift from Jeremy Luban (Addgene plasmid # 132939 ; http://n2t.net/addgene:132939 ; RRID:Addgene_132939) -
For your References section:
Cyclophilin A protects HIV-1 from restriction by human TRIM5alpha. Kim K, Dauphin A, Komurlu S, McCauley SM, Yurkovetskiy L, Carbone C, Diehl WE, Strambio-De-Castillia C, Campbell EM, Luban J. Nat Microbiol. 2019 Dec;4(12):2044-2051. doi: 10.1038/s41564-019-0592-5. Epub 2019 Oct 21. 10.1038/s41564-019-0592-5 PubMed 31636416