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Addgene

p8.9 NdeltaSB D185K/D186L
(Plasmid #132931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p8.9 NdeltaSB
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gag and pol D185K/D186L
  • Species
    HIV
  • Mutation
    D185K/D186L

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p8.9 NdeltaSB D185K/D186L was a gift from Jeremy Luban (Addgene plasmid # 132931 ; http://n2t.net/addgene:132931 ; RRID:Addgene_132931)

  • For your References section:

    Inhibition of HIV-1 infection by TNPO3 depletion is determined by capsid and detectable after viral cDNA enters the nucleus. De Iaco A, Luban J. Retrovirology. 2011 Dec 6;8:98. doi: 10.1186/1742-4690-8-98. 10.1186/1742-4690-8-98 PubMed 22145813
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