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Addgene

LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS
(Plasmid #132927)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 132927 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221 P4r-P3r
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2664
  • Total vector size (bp) 6876
  • Vector type
    CRISPR, Synthetic Biology ; pMAGIC Gateway Entry plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized SpCas9
  • Alt name
    S. pyogenes CRISPR-Cas9
  • Alt name
    Cas9
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    4212

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SpCas9 was PCR'd from lentiCRISPRv2 (Addgene #52961) and inserted into PmeI/SrFI digested KJ901 (Addgene #121835).

This entry plasmid is compatible with pMAGIC cloning platform (PMID: 30590691). The 2x-NLS SpCas9 gene encoded in this plasmid does not contain a stop codon. SpCas9 gRNAs can be expressed from plasmids KK701 (Addgene #121842), LF701 (Addgene #121817), or LG002 (Addgene # 121816).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS was a gift from Christopher Newgard (Addgene plasmid # 132927 ; http://n2t.net/addgene:132927 ; RRID:Addgene_132927)
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